Pediatric Hematology/Oncology and ImmunopathologyPediatric Hematology/Oncology and ImmunopathologyВопросы гематологии/онкологии и иммунопатологии в педиатрии1726-17082414-9314Fund Doctors, Innovations, Science for Children13510.24287/1726-1708-2016-15-4-66-73ONCOLOGYОНКОЛОГИЯPrimary diagnostics and assessment of minimal disseminated and minimal residual disease in Burkitt lymphoma/leukemia by detection of c-MYC-IgH rearrangements with long-distance polymerase chain reactionПервичная диагностика и оценка минимальной диссеминированной и минимальной остаточной болезни путем определения перестройки c-MYC-IgH с помощью полимеразной цепной реакции длинных фрагментов при лимфоме/лейкозе БеркиттаLavrinenkoVictoria A.ЛавриненкоВиктория Александровна
Russian Federation
lavrinenkovictoria@gmail.comVolochnikElena V.ВолочникЕлена Викторовна
Russian Federation
valochnikalena@gmail.comFedorovaAlina S.ФёдороваАлина Степановна
Russian Federation
alina_fedorova@list.ruAleinikovaOlga V.АлейниковаОльга Витальевна
Russian Federation
aleinikova2004@mail.ruBelarusian Research Center for Pediatric Oncology, Hematology and ImmunologyРеспубликанский научно-практический центр детской онкологии, гематологии и иммунологии Минздрава Республики Беларусь19122016154667319092018Copyright ©; 2016, «D. Rogachev NMRCPHOI»Copyright ©; 2016, ФГБУ «НМИЦ ДГОИ им. Дмитрия Рогачева» Минздрава России2016«D. Rogachev NMRCPHOI»ФГБУ «НМИЦ ДГОИ им. Дмитрия Рогачева» Минздрава Россииhttps://creativecommons.org/licenses/by/4.0https://hemoncim.com/jour/article/view/135

The aim of this study was to identify с-MYC-IgH with long-distance polymerase chain reaction (LD-PCR) for Burkitt's lymphoma/ leukemia diagnosis, to compare LD-PCR with cytogenetic methods and to evaluate the possibility of LD-PCR application for minimal disseminated (MdD) and minimal residual disease (MRD) detection. с-MYC-IgH rearrangements were detected in 63,6% of the 55 patients at the age 2-23 years. In comparison to G-banding, LD-PCR was possible to perform for all patients, however LD-PCR detected с-MYC-IgH only in 71,1% of the patients with translocation t (8;14)(q24;q32). с-MYC-IgH rearrangements were found by LD-PCR in 2 of 5 (40%) of patients who were negative by FISH and G-banding. Combination of these methods allowed to reveal of c-MYC rearrangements in 94,5% of the patients. MDD was identified in 11,1% patient with stage III and in all patients with stage IV both negative and positive by standard morphological analysis. LD-PCR could be used for primary diagnosis of Burkitt's lymphoma/leukemia and MDD detection. At the same time, LD-PCR is less informative for MRD evaluation because of low sensitivity and possibility to obtain only qualitative results.

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