Pediatric Hematology/Oncology and ImmunopathologyPediatric Hematology/Oncology and ImmunopathologyВопросы гематологии/онкологии и иммунопатологии в педиатрии1726-17082414-9314Fund Doctors, Innovations, Science for Children15910.24287/1726-1708-2016-15-2-32-39СтатьиThe use of a spatial thrombin generation method for assessment of platelet procoagulant activity after platelet concentrate transfusion in childrenИспользование метода пространственной генерации тромбина для оценки прокоагулянтной активности тромбоцитов после трансфузии тромбоконцентрата у детейKol'tsovaEkaterina M.КольцоваЕкатерина Михайловна
Russian Federation
ekaterina_koltsova@bk.ruBalandinaJunior AnnaБаландинаАнна Николаевна
Russian Federation
a_balandina@inbox.ruDeminaIrina A.ДеминаИрина Андреевна
Russian Federation
idemina@mail.ruRadyginaSvetlana A.РадыгинаСветлана Анатольевна
Russian Federation
svetlana.radygina@fccho-moscow.ruAtaullakhanovFazoil I.АтауллахановФазоил Инноятович
Russian Federation
ataullakhanov.fazly@gmail.comBalashovDmitrii N.БалашовДмитрий Николаевич
Russian Federation
dmitry.balashov@fccho-moscow.ruPanteleevMikhail A.ПантелеевМихаил Александрович
Russian Federation
mapanteleev@yandex.ruFederal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry RogachevФедеральный научно-клинический центр детской гематологии, онкологии и иммунологии им. Дмитрия Рогачева Минздрава РоссииCentre for Theoretical Problems of Physico-Chemical Pharmacology, Russian Academy of SciencesЦентр теоретических проблем физико-химической фармакологии РАНM.V.Lomonosov Moscow State UniversityМосковский государственный университет им. М.В.ЛомоносоваMoscow Institute of Physics and Technology (State University)Московский физико-технический институт (государственный университет)19062016152323919092018Copyright ©; 2016, «D. Rogachev NMRCPHOI»Copyright ©; 2016, ФГБУ «НМИЦ ДГОИ им. Дмитрия Рогачева» Минздрава России2016«D. Rogachev NMRCPHOI»ФГБУ «НМИЦ ДГОИ им. Дмитрия Рогачева» Минздрава Россииhttps://creativecommons.org/licenses/by/4.0https://hemoncim.com/jour/article/view/159

Assessment of the effectiveness of platelet concentrate transfusion after hematopoietic stem cell transplantation is based on calculation of the amount of platelets and does not take into account their functional activity. The article offers an integral study of the state of hemostasis in 10 children (aged 3 to 16 years, median 11 years) before and after platelet concentrate transfusion with the use of tests for assessment of platelet functional activity and thrombin generation in space (thrombodynamics-4D). Two hours after platelet concentrate transfusion the amount of platelet increased from 8.0 ± 6.6 to 52.3 ± 25.2 X 109/L. Secretion of platelet dense granules (1.37 ± 0.48, norm 3.52 ± 1.14 units) and a-granules (5969 ± 2366, norm 11787 ± 4639 units), activated (385 ± 167, norm 1279 ± 682 units) and non-activated glycoprotein IIb/IIIa (3179 ± 1609, norm 5557 ± 2017 units) before transfusion was lower as compared with values of healthy volunteers and did not change after transfusion. A characteristic for healthy volunteers «thrombin wave» in studying thrombodynamics-4D was not formed in samples of patients both before and after transfusion. After platelet concentrate transfusion, an increase of the clot growth rate from 25 ± 8 to 32 ± 4 pm/min (p < 0.005) and thrombin reaction product from 124 ± 49 to 209 ± 38 pM*mm was observed (p < 0.001). The increased amounts of thrombin are indicative of improvement of blood coagulation after platelet concentrate transfusion, but parameters of platelet functional activity and thrombodynamics-4D do not reach normal values.

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