Pediatric Hematology/Oncology and ImmunopathologyPediatric Hematology/Oncology and ImmunopathologyВопросы гематологии/онкологии и иммунопатологии в педиатрии1726-17082414-9314Fund Doctors, Innovations, Science for Children49910.24287/1726-1708-2021-20-1-207-217LITERATURE REVIEWОБЗОР ЛИТЕРАТУРЫAnalysis of platelet RNA: a non-invasive method for studying the expression of tumor genesАнализ тромбоцитарной РНК: неинвазивный метод изучения экспрессии опухолевых геновhttps://orcid.org/0000-0002-9366-3449TesakovI. P.ТесаковИ. П.
Russian Federation

Moscow

Москва

https://orcid.org/0000-0003-0211-6325MartyanovA. A.МартьяновА. А.
Russian Federation

Moscow

Москва

https://orcid.org/0000-0003-1308-8622DruiA. E.ДруйА. Е.
Russian Federation

Moscow

Москва

https://orcid.org/0000-0003-4720-7319SveshnikovaA. N.СвешниковаА. Н.
Russian Federation

head of the Intracellular signalling and systems biology laboratory,

30 Srednyaya Kalitnikovskaya st., 109029 Moscow

заведующая лабораторией внутриклеточной сигнализации и системной биологии,

109029, Москва, ул. Средняя Калитниковская, 30

a.sveshnikova@physics.msu.ruLomonosov Moscow State UniversityФГБОУ ВО «Московский государственный университет им. М.В. Ломоносова», МоскваDmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Ministry of Healthcare of the Russian FederationФГБУ «Национальный медицинский исследовательский центр детской гематологии, онкологии и иммунологии им. Дмитрия Рогачева» Минздрава РоссииCenter for Theoretical Problems of Physicochemical Pharmacology, RASФГБУН «Центр теоретических проблем физико-химической фармакологии» РАН210420212012072172104202121042021Copyright ©; 2021, «D. Rogachev NMRCPHOI»Copyright ©; 2021, ФГБУ «НМИЦ ДГОИ им. Дмитрия Рогачева» Минздрава России2021«D. Rogachev NMRCPHOI»ФГБУ «НМИЦ ДГОИ им. Дмитрия Рогачева» Минздрава Россииhttps://creativecommons.org/licenses/by/4.0https://hemoncim.com/jour/article/view/499

Nowadays much attention is paid to non-invasive methods of cancer diagnostics and monitoring. One of the promising methods is the sequencing of platelet RNA (tumor-educated platelets), in which, as it was previously established, the mRNA repertoire changes in various oncological diseases. Thus, platelets can contain information about the molecular genetic characteristics of tumor. This review summarizes the current understanding of the mechanisms of interaction between tumor cells and platelets, and also discusses the possibilities of using platelet transcriptome analysis methods for diagnosing and assessing the dynamics of the tumor state, in particular, in response to therapy, as well as the current limitations for a wide range of introduction of this method into clinical practice. 

В настоящее время много внимания уделяется неинвазивным методам диагностики и мониторинга онкологических заболеваний. Одним из перспективных методов является секвенирование РНК тромбоцитов, «обученных» опухолью (tumor-educated platelets), в которых, как было ранее установлено, изменяется мРНК-репертуар при различных онкологических заболеваниях. Таким образом, тромбоциты могут содержать информацию о молекулярно-генетических характеристиках опухоли. В настоящем обзоре собраны современные представления о механизмах взаимодействия между опухолевыми клетками и тромбоцитами, а также рассмотрены возможности применения методов анализа транскриптома тромбоцитов для диагностики и оценки динамики состояния опухоли, в частности ответа на проводимую терапию, а также существующие на сегодняшний день ограничения для широкого внедрения этого метода в клиническую практику. 

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