Pediatric Hematology/Oncology and ImmunopathologyPediatric Hematology/Oncology and ImmunopathologyВопросы гематологии/онкологии и иммунопатологии в педиатрии1726-17082414-9314Fund Doctors, Innovations, Science for Children62310.24287/1726-1708-2022-21-2-112-117CLINICAL OBSERVATIONSКЛИНИЧЕСКИЕ НАБЛЮДЕНИЯThe use of adoptive cell therapy for the treatment of SARS-CoV-2 in a patient after allogeneic hematopoietic stem cell transplantationИспользование адоптивной клеточной терапии для лечения SARS-CoV-2 у пациента после аллогенной трансплантации гемопоэтических стволовых клетокhttps://orcid.org/0000-0002-0799-1025LeontyevaM. E.ЛеонтьеваМ. Е.
Russian Federation

Marina Е. Leontyeva, a resident

1 Samory Mashela St., Moscow 117997

Леонтьева Марина Евгеньевна, врач-ординатор

117997, Москва, ул. Саморы Машела, 1

me-15.leontjeva@yandex.ruhttps://orcid.org/0000-0003-0319-3699SultanovaE. R.СултановаЭ. Р.
Russian Federation

Moscow

Москва

https://orcid.org/0000-0002-7696-1153RadyginaS. A.РадыгинаС. А.
Russian Federation

Moscow

Москва

https://orcid.org/0000-0002-0566-053XSkvortsovaYu. V.СкворцоваЮ. В.
Russian Federation

Moscow

Москва

https://orcid.org/0000-0003-3513-8299MuzalevskiyYa. O.МузалевскийЯ. О.
Russian Federation

Moscow

Москва

https://orcid.org/0000-0003-4767-5382KurnikovaE. E.КурниковаЕ. Е.
Russian Federation

Moscow

Москва

https://orcid.org/0000-0002-6148-7209PershinD. E.ПершинД. Е.
Russian Federation

Moscow

Москва

https://orcid.org/0000-0003-1735-0093MaschanM. A.МасчанМ. А.
Russian Federation

Moscow

Москва

https://orcid.org/0000-0003-2689-0569BalashovD. N.БалашовД. Н.
Russian Federation

Moscow

Москва

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian FederationФГБУ «Национальный медицинский исследовательский центр детской гематологии, онкологии и иммунологии им. Дмитрия Рогачева» Минздрава России080720222121121172406202224062022Copyright ©; 2025, «D. Rogachev NMRCPHOI»Copyright ©; 2025, ФГБУ «НМИЦ ДГОИ им. Дмитрия Рогачева» Минздрава России2025«D. Rogachev NMRCPHOI»ФГБУ «НМИЦ ДГОИ им. Дмитрия Рогачева» Минздрава Россииhttps://creativecommons.org/licenses/by/4.0https://hemoncim.com/jour/article/view/623

   The COVID-19 pandemic continues to be a major public health threat worldwide. The course of this disease in immunocompromised patients is significantly different from that in healthy subjects, which is associated with the impossibility of virus elimination through their own adaptive immune response. Delayed immune reconstitution after hematopoietic stem cell transplantation (which may take months after the procedure) increases the risk of life-threatening COVID-19 infection necessitating a search for and application of new methods of treatment. T lymphocytes are critically important for viral infection control, and are necessary both for the direct elimination of Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation. The patient's parents gave consent to the use of their child's data, including photographs, for research purposes and in publications.

   Пандемия новой коронавирусной инфекции (COVID-19), вызванная SARS‐CoV‐2, по-прежнему является серьезной угрозой общественному здоровью во всем мире. Течение данного заболевания у иммунокомпрометированных пациентов значительно отличается, что связано с невозможностью элиминации вируса посредством собственного адаптивного иммунного ответа. Отсроченная на месяцы после трансплантации гемопоэтических стволовых клеток иммунная реконституция увеличивает риск жизнеугрожающего течения COVID-19. Данный факт требует поиска и применения новых методов лечения. Важнейшим инструментом контроля вирусных инфекций являются Т-лимфоциты, которые нужны как для непосредственной элиминации вируса, так и для инициации каскада последовательных клеточных взаимодействий, необходимых для формирования специфического адаптивного ответа. В данной работе представлен клинический случай проведения клеточной терапии COVID-19 с использованием вирусспецифических Т-лимфоцитов к SARS-CoV-2. Родители пациентки дали согласие на использование информации, в том числе фотографий ребенка, в научных исследованиях и публикациях.

SARS-CoV-2coronavirus infectionCOVID-19virus-specific T lymphocyteshematopoietic stem cell transplantationallogeneic hematopoietic stem cell transplantationpneumoniaprimary immunodeficiencySARS‐CoV‐2коронавирусная инфекцияCOVID-19вирусспецифические Т-лимфоцитытрансплантация гемопоэтических стволовых клетокаллогенная трансплантация гемопоэтических стволовых клетокпневмонияпервичный иммунодефицитNot specifiedНе указан1. Campos D. M. O., Fulco U. L., de Oliveira C. B. S., Oliveira J. I. N. SARS‐CoV‐2 virus infection: Targets and antiviral pharmacological strategies. J Evid Based Med 2020; 13 (4): 255–60. DOI: 10.1111/jebm.12414Campos D. M. O., Fulco U. L., de Oliveira C. B. S., Oliveira J. I. N. SARS‐CoV‐2 virus infection: Targets and antiviral pharmacological strategies. J Evid Based Med 2020; 13 (4): 255–60. DOI: 10.1111/jebm.124142. Wang M. Y., Zhao R., Gao L. J., Gao X. F., Wang D. P., Cao J. M. SARS-CoV-2: Structure, Biology, and Structure-Based Therapeutics Development. Front Cell Infect Microbiol 2020; 10: 587269. DOI: 10.3389/fcimb.2020.587269Wang M. Y., Zhao R., Gao L. J., Gao X. F., Wang D. P., Cao J. M. SARS-CoV-2: Structure, Biology, and Structure-Based Therapeutics Development. Front Cell Infect Microbiol 2020; 10: 587269. DOI: 10.3389/fcimb.2020.5872693. Anka A. U., Tahir M. I., Abubakar S. D., Alsabbagh M., Zian Z., Hamedifar H., et аl. Coronavirus disease 2019 (COVID-19): An overview of the immunopathology, serological diagnosis and management. Scand J Immunol 2021; 93 (4): e12998. DOI: 10.1111/sji.12998Anka A. U., Tahir M. I., Abubakar S. D., Alsabbagh M., Zian Z., Hamedifar H., et аl. Coronavirus disease 2019 (COVID-19): An overview of the immunopathology, serological diagnosis and management. Scand J Immunol 2021; 93 (4): e12998. DOI: 10.1111/sji.129984. Di Piazza A.T., Graham B. S., Ruckwardt T. J. T cell immunity to SARS-CoV-2 following natural infection and vaccination. Biochem Biophys Res Commun 2021; 538: 211–7. DOI: 10.1016/j.bbrc.2020.10.060Di Piazza A.T., Graham B. S., Ruckwardt T. J. T cell immunity to SARS-CoV-2 following natural infection and vaccination. Biochem Biophys Res Commun 2021; 538: 211–7. DOI: 10.1016/j.bbrc.2020.10.0605. Shi Y., Wang Y., Shao C., Huang J., Gan J., Huang X., et аl. COVID-19 infection: the perspectives on immune responses. Cell Death Differ 2020; 27 (5): 1451–4. DOI: 10.1038/s41418-020-0530-3Shi Y., Wang Y., Shao C., Huang J., Gan J., Huang X., et аl. COVID-19 infection: the perspectives on immune responses. Cell Death Differ 2020; 27 (5): 1451–4. DOI: 10.1038/s41418-020-0530-36. Liang W., Guan W., Chen R., Wang W., Li J., Xu K., et аl. Cancer patients in SARS-CoV-2 infection: a nationwide analysis in China. Lancet Oncol 2020; 21 (3): 335–7. DOI: 10.1016/S1470-2045(20)30096-6Liang W., Guan W., Chen R., Wang W., Li J., Xu K., et аl. Cancer patients in SARS-CoV-2 infection: a nationwide analysis in China. Lancet Oncol 2020; 21 (3): 335–7. DOI: 10.1016/S1470-2045(20)30096-67. Balashov D., Trakhtman P., Livshits A., Kovalenko I., Tereshenko G., Solopova G., et аl. SARS-CoV-2 convalescent plasma therapy in pediatric patient after hematopoietic stem cell transplantation. Transfus Apher Sci 2021; 60 (1): 102983. DOI: 10.1016/j.transci.2020.102983Balashov D., Trakhtman P., Livshits A., Kovalenko I., Tereshenko G., Solopova G., et аl. SARS-CoV-2 convalescent plasma therapy in pediatric patient after hematopoietic stem cell transplantation. Transfus Apher Sci 2021; 60 (1): 102983. DOI: 10.1016/j.transci.2020.1029838. Papadopoulou A., Gerdemann U., Katari U. L., Tzannou I., Liu H., Martinez C., et аl. Activity of broad-spectrum T cells as treatment for AdV, EBV, CMV, BKV, and HHV6 infections after HSCT. Sci Transl Med 2014; 6 (242): 242ra83. DOI: 10.1126/scitranslmed.3008825Papadopoulou A., Gerdemann U., Katari U. L., Tzannou I., Liu H., Martinez C., et аl. Activity of broad-spectrum T cells as treatment for AdV, EBV, CMV, BKV, and HHV6 infections after HSCT. Sci Transl Med 2014; 6 (242): 242ra83. DOI: 10.1126/scitranslmed.30088259. Barrett A. J., Prockop S., Bollard C. M. Virus-Specific T Cells: Broadening Applicability. Biol Blood Marrow Transplant 2018; 24 (1): 13–8. DOI: 10.1016/j.bbmt.2017.10.004Barrett A. J., Prockop S., Bollard C. M. Virus-Specific T Cells: Broadening Applicability. Biol Blood Marrow Transplant 2018; 24 (1): 13–8. DOI: 10.1016/j.bbmt.2017.10.00410. Kállay K., Kassa C., Réti M., Karászi É., Sinkó J., Goda V., et аl. Early Experience With CliniMACS Prodigy CCS (IFN-gamma) System in Selection of Virus-specific T Cells From Third-party Donors for Pediatric Patients With Severe Viral Infections After Hematopoietic Stem Cell Transplantation. J Immunother 2018; 41 (3): 158–63. DOI: 10.1097/CJI.0000000000000197Kállay K., Kassa C., Réti M., Karászi É., Sinkó J., Goda V., et аl. Early Experience With CliniMACS Prodigy CCS (IFN-gamma) System in Selection of Virus-specific T Cells From Third-party Donors for Pediatric Patients With Severe Viral Infections After Hematopoietic Stem Cell Transplantation. J Immunother 2018; 41 (3): 158–63. DOI: 10.1097/CJI.000000000000019711. Tzannou I., Papadopoulou A., Naik S., Leung K., Martinez C. A., Ramos C. A., еt al. Off-the-Shelf Virus Specific T Cells to Treat BK Virus, Human Herpesvirus 6, Cytomegalovirus, Epstein-Barr Virus, and Adenovirus Infections After Allogeneic Hematopoietic Stem-Cell Transplantation. J Clin Oncol 2017; 35 (31): 3547–57. DOI: 10.1200/JCO.2017.73.0655Tzannou I., Papadopoulou A., Naik S., Leung K., Martinez C. A., Ramos C. A., еt al. Off-the-Shelf Virus Specific T Cells to Treat BK Virus, Human Herpesvirus 6, Cytomegalovirus, Epstein-Barr Virus, and Adenovirus Infections After Allogeneic Hematopoietic Stem-Cell Transplantation. J Clin Oncol 2017; 35 (31): 3547–57. DOI: 10.1200/JCO.2017.73.065512. Naik S., Nicholas S. K., Martinez C. A., Leen A. M., Hanley P. J., Gottschalk S. M., et аl. Adoptive immunotherapy for primary immunodeficiency disorders with virus-specific T lymphocytes. J Allergy Clin Immunol 2016; 137 (5): 1498–505. e1. DOI: 10.1016/j.jaci.2015.12.1311Naik S., Nicholas S. K., Martinez C. A., Leen A. M., Hanley P. J., Gottschalk S. M., et аl. Adoptive immunotherapy for primary immunodeficiency disorders with virus-specific T lymphocytes. J Allergy Clin Immunol 2016; 137 (5): 1498–505. e1. DOI: 10.1016/j.jaci.2015.12.131113. McLaughlin L. P., Bollard C. M., Keller M. D. Adoptive T Cell Therapy for Epstein–Barr Virus Complications in Patients With Primary Immunodeficiency Disorders. Front Immunol 2018; 9: 556. DOI: 10.3389/fimmu.2018.00556McLaughlin L. P., Bollard C. M., Keller M. D. Adoptive T Cell Therapy for Epstein–Barr Virus Complications in Patients With Primary Immunodeficiency Disorders. Front Immunol 2018; 9: 556. DOI: 10.3389/fimmu.2018.0055614. Al Hamed R., Bazarbachi A. H., Mohty M. Epstein–Barr virus-related post-transplant lymphoproliferative disease (EBV-PTLD) in the setting of allogeneic stem cell transplantation: a comprehensive review from pathogenesis to forthcoming treatment modalities. Bone Marrow Transplant 2020; 55 (1): 25–39. DOI: 10.1038/s41409-019-0548-7Al Hamed R., Bazarbachi A. H., Mohty M. Epstein–Barr virus-related post-transplant lymphoproliferative disease (EBV-PTLD) in the setting of allogeneic stem cell transplantation: a comprehensive review from pathogenesis to forthcoming treatment modalities. Bone Marrow Transplant 2020; 55 (1): 25–39. DOI: 10.1038/s41409-019-0548-7