Pediatric Hematology/Oncology and ImmunopathologyPediatric Hematology/Oncology and ImmunopathologyВопросы гематологии/онкологии и иммунопатологии в педиатрии1726-17082414-9314Fund Doctors, Innovations, Science for Children7610.24287/1726-1708-2017-16-1-10-21СтатьиHerpesvirus infection following allogenic hematopoietic stem cell transplantation with TCRαβ and CD19 depletion: risk factors and outcomeГерпес-вирусные инфекции у реципиентов аллогенной трансплантации гемопоэтических стволовых клеток с TCRaβи CD19 деплецией: факторы риска и прогнозBogoyavlenskayaA. A.БогоявленскаяА. А.
Russian Federation

MD, hematologist, Department of hematopoietic stem cell Transfusion

Russia 117997, Moscow, Samory Mashela st., 1

Tel.: +7 (495) 287-6570, ext. 7537

analecs@gmail.comLaberkoA. L.ЛаберкоА. Л.
Russian Federation
ShelikhovaL. N.ШелиховаЛ. Н.
Russian Federation
ShekhovtsovaZ. B.ШеховцоваЖ. Б.
Russian Federation
BalashovD. N.БалашовД. Н.
Russian Federation
VoroninK. A.ВоронинК. А.
Russian Federation
KurnikovaE. E.КурниковаЕ. Е.
Russian Federation
BoyakovaE. V.БояковаЕ. В.
Russian Federation
RaykinaE. V.РайкинаЕ. В.
Russian Federation
BrilliantovaV. V.БриллиантоваВ. В.
Russian Federation
PirumovaR. P.ПирумоваР. П.
Russian Federation
MaschanM. A.МасчанМ. А.
Russian Federation
National Reseach Practicle Center of Pediatric, Oncology, and Immunology named after Dmitry RogachevФГБУ «Национальный научно-практический центр детской гематологии, онкологии и иммунологии им. Дмитрия Рогачева» Минздрава России, Москва10022017161102110082018Copyright ©; 2017, «D. Rogachev NMRCPHOI»Copyright ©; 2017, ФГБУ «НМИЦ ДГОИ им. Дмитрия Рогачева» Минздрава России2017«D. Rogachev NMRCPHOI»ФГБУ «НМИЦ ДГОИ им. Дмитрия Рогачева» Минздрава Россииhttps://creativecommons.org/licenses/by/4.0https://hemoncim.com/jour/article/view/76

In this study we evaluated incidence and risk factors of Herpesvirus infections – Cytomegalovirus (CMV) и Epstein–Barr (EBV) – in recipients of allogenic hematopoietic stem cell transplantation (alloHSCT) with TCRαβ and CD19 depletion of graft. The usage of this method results to graft preservation of non-alloreactive TCRgd lymphocytes, leading to decresed risks of graft versus host disease (GVHD) with adequate antiviral control. We estimated the outcomes of 182 HSCT from haploidentical and unrelated donors in patients younger than 23 years old with malignant anf nonmalignant disoders. The cumulative incidence (Cum Inc) of CMV infection was 51% (95% CI 44–60). By uni- and multivariate analyzes was found the significant influence on CMV-viremia of acute GVHD grade 2–4, recipient’s seropositivity before HSCT and patient’s malignant disoder. Cum Inc of EBV infection was 33% (95% CI 26–42). The risk factor of EBV viremia was acute GVHD grade 2–4. The incidence of CMV-disease was 6% (11 patients), EBV-related posttransplant lymphoproliferative disoder (PTLD) 0,5% (1 patient). CMV and EBV viremia did not decrease survival after HSCT. TCRαβ and CD19 depletion of graft was associated with significant risk of CMV viremia in patients after alloHSCT, but did not influence survival and transplant related mortality, and with extremely low risk of PTLD development.

herpesvirus (CMV, EBV)TCRαβ depletionallogenic stem cell transplantationгерпес-вирусные инфекцииTCRaβ-деплецияаллогенная ТГСК1. Bertaina A., Merli P., Rutella S., Pagliara D., Bernardo M.E., Masetti R. et al. HLA-haploidentical stem cell transplantation after removal of + T and B cells in children with nonmalignant disorders. Blood. 2014;124(5):822-826.Bertaina A., Merli P., Rutella S., Pagliara D., Bernardo M.E., Masetti R. et al. HLA-haploidentical stem cell transplantation after removal of + T and B cells in children with nonmalignant disorders. Blood. 2014;124(5):822-826.2. Balashov D., Shcherbina A., Maschan M., Trakhtman P., Skvortsova Y., Shelikhova L. et al. Single-Center Experience of Unrelated and Haploidentical Stem Cell Transplantation with TCRalphabeta and CD19 Depletion in Children with Primary Immunodeficiency Syndromes. Biol Blood Marrow Transplant. 2015;21:1955-1962.Balashov D., Shcherbina A., Maschan M., Trakhtman P., Skvortsova Y., Shelikhova L. et al. Single-Center Experience of Unrelated and Haploidentical Stem Cell Transplantation with TCRalphabeta and CD19 Depletion in Children with Primary Immunodeficiency Syndromes. Biol Blood Marrow Transplant. 2015;21:1955-1962.3. Maschan M., Shelikhova L., Ilushina M., Kurnikova E., Boyakova E., Balashov D. et al. TCR-alpha/beta and CD19 depletion and treosulfan-based conditioning regimen in unrelated and haploidentical transplantation in children with acute myeloid leukemia. Bone marrow transplant. 2016;51:668-674.Maschan M., Shelikhova L., Ilushina M., Kurnikova E., Boyakova E., Balashov D. et al. TCR-alpha/beta and CD19 depletion and treosulfan-based conditioning regimen in unrelated and haploidentical transplantation in children with acute myeloid leukemia. Bone marrow transplant. 2016;51:668-674.4. Peterson P.K., McGlave P., Ramsay N.K., Rhame F., Cohen E., Perry GS. 3rd et al. A prospective study of infectious diseases following bone marrow transplantation: emergence of Aspergillus and Cytomegalovirus as the major causes of mortality. Infect Control. 1983;4: 81-89.Peterson P.K., McGlave P., Ramsay N.K., Rhame F., Cohen E., Perry GS. 3rd et al. A prospective study of infectious diseases following bone marrow transplantation: emergence of Aspergillus and Cytomegalovirus as the major causes of mortality. Infect Control. 1983;4: 81-89.5. Ljungman P. The role of cytomegalovirus serostatus on outcome of hematopoietic stem cell transplantation. Curr Opin Hematol. 2014;21:466-469.Ljungman P. The role of cytomegalovirus serostatus on outcome of hematopoietic stem cell transplantation. Curr Opin Hematol. 2014;21:466-469.6. Boeckh M., Nichols W.G. The impact of cytomegalovirus serostatus of donor and recipient before hematopoietic stem cell transplantation in the era of antiviral prophylaxis and preemptive therapy. Blood. 2004;103:2003-2008.Boeckh M., Nichols W.G. The impact of cytomegalovirus serostatus of donor and recipient before hematopoietic stem cell transplantation in the era of antiviral prophylaxis and preemptive therapy. Blood. 2004;103:2003-2008.7. Jain N.A., Lu K., Ito S., Muranski P., Hourigan C.S., Haggerty J. et al. The clinical and financial burden of pre-emptive management of cytomegalovirus disease after allogeneic stem cell transplantation-implications for preventative treatment approaches. Cytotherapy. 2014;16:927-933.Jain N.A., Lu K., Ito S., Muranski P., Hourigan C.S., Haggerty J. et al. The clinical and financial burden of pre-emptive management of cytomegalovirus disease after allogeneic stem cell transplantation-implications for preventative treatment approaches. Cytotherapy. 2014;16:927-933.8. Davis Z.B., Cooley S.A., Cichocki F., Felices M., Wangen R., Luo X. et al. Adaptive Natural Killer Cell and Killer Cell Immunoglobulin-Like Receptor-Expressing T Cell Responses are Induced by Cytomegalovirus and Are Associated with Protection against Cytomegalovirus Reactivation after Allogeneic Donor Hematopoietic Cell Transplantation. Biol Blood Marrow Transplant. 2015;21:1653-1662.Davis Z.B., Cooley S.A., Cichocki F., Felices M., Wangen R., Luo X. et al. Adaptive Natural Killer Cell and Killer Cell Immunoglobulin-Like Receptor-Expressing T Cell Responses are Induced by Cytomegalovirus and Are Associated with Protection against Cytomegalovirus Reactivation after Allogeneic Donor Hematopoietic Cell Transplantation. Biol Blood Marrow Transplant. 2015;21:1653-1662.9. Couriel D., Canosa J., Engler H., Collins A., Dunbar C., Barrett A.J. Early reactivation of cytomegalovirus and high risk of interstitial pneumonitis following T-depleted BMT for adults with hematological malignancies. Bone Marrow Transplant. 1996;18:347-353.Couriel D., Canosa J., Engler H., Collins A., Dunbar C., Barrett A.J. Early reactivation of cytomegalovirus and high risk of interstitial pneumonitis following T-depleted BMT for adults with hematological malignancies. Bone Marrow Transplant. 1996;18:347-353.10. Lugthart G., van Ostaijen-Ten Dam M.M., Jol-van der Zijde C.M., van Holten T.C., Kester M.G., Heemskerk M.H. et al. Early cytomegalovirus reactivation leaves a specific and dynamic imprint on the reconstituting T cell compartment long-term after hematopoietic stem cell transplantation. Biol Blood Marrow Transplant. 2014;20(5):655-661.Lugthart G., van Ostaijen-Ten Dam M.M., Jol-van der Zijde C.M., van Holten T.C., Kester M.G., Heemskerk M.H. et al. Early cytomegalovirus reactivation leaves a specific and dynamic imprint on the reconstituting T cell compartment long-term after hematopoietic stem cell transplantation. Biol Blood Marrow Transplant. 2014;20(5):655-661.11. Miller W., Flynn P., McCullough J., Balfour H.H. Jr, Goldman A., Haake R. et al. Cytomegalovirus infection after bone marrow transplantation: an association with acute graft-v-host disease. Blood. 1986;67:1162-1167.Miller W., Flynn P., McCullough J., Balfour H.H. Jr, Goldman A., Haake R. et al. Cytomegalovirus infection after bone marrow transplantation: an association with acute graft-v-host disease. Blood. 1986;67:1162-1167.12. Yoon J.H., Lee S., Kim H.J., Jeon Y.W., Lee S.E., Cho B.S. et al. Impact of cytomegalovirus reactivation on relapse and survival in patients with acute leukemia who received allogeneic hematopoietic stem cell transplantation in first remission. Oncotarget. 2016;7:17230-17241.Yoon J.H., Lee S., Kim H.J., Jeon Y.W., Lee S.E., Cho B.S. et al. Impact of cytomegalovirus reactivation on relapse and survival in patients with acute leukemia who received allogeneic hematopoietic stem cell transplantation in first remission. Oncotarget. 2016;7:17230-17241.13. Wu J.L., Ma H.Y., Lu C.Y., Chen J.M,. Lee P.I., Jou S.T. et al. Risk factors and outcomes of cytomegalovirus viremia in pediatric hematopoietic stem cell transplantation patients. J Microbiol Immunol Infect. 2015.Wu J.L., Ma H.Y., Lu C.Y., Chen J.M,. Lee P.I., Jou S.T. et al. Risk factors and outcomes of cytomegalovirus viremia in pediatric hematopoietic stem cell transplantation patients. J Microbiol Immunol Infect. 2015.14. Kalra A., Williamson T., Daly A., Savoie M.L., Stewart D.A., Khan F. et al. Impact of Donor and Recipient Cytomegalovirus Serostatus on Outcomes of Antithymocyte Globulin-Conditioned Hematopoietic Cell Transplantation. Biol Blood Marrow Transplant. 2016;22:1654-1663.Kalra A., Williamson T., Daly A., Savoie M.L., Stewart D.A., Khan F. et al. Impact of Donor and Recipient Cytomegalovirus Serostatus on Outcomes of Antithymocyte Globulin-Conditioned Hematopoietic Cell Transplantation. Biol Blood Marrow Transplant. 2016;22:1654-1663.15. Ljungman P., Brand R., Hoek J., de la Camara R., Cordonnier C., Einsele H. et al. Donor cytomegalovirus status influences the outcome of allogeneic stem cell transplant: a study by the European group for blood and marrow transplantation. Clin Infect Dis. 2014;59:473-481.Ljungman P., Brand R., Hoek J., de la Camara R., Cordonnier C., Einsele H. et al. Donor cytomegalovirus status influences the outcome of allogeneic stem cell transplant: a study by the European group for blood and marrow transplantation. Clin Infect Dis. 2014;59:473-481.16. Elmaagacli A.H., Steckel N.K., Koldehoff M., Hegerfeldt Y., Trenschel R., Ditschkowski M. et al. Early human cytomegalovirus replication after transplantation is associated with a decreased relapse risk: evidence for a putative virus-versus-leukemia effect in acute myeloid leukemia patients. Blood. 2011;118:1402-1412.Elmaagacli A.H., Steckel N.K., Koldehoff M., Hegerfeldt Y., Trenschel R., Ditschkowski M. et al. Early human cytomegalovirus replication after transplantation is associated with a decreased relapse risk: evidence for a putative virus-versus-leukemia effect in acute myeloid leukemia patients. Blood. 2011;118:1402-1412.17. Manjappa S., Bhamidipati P.K., Stokerl-Goldstein K.E., DiPersio J.F., Uy G.L., Westervelt P. et al. Protective effect of cytomegalovirus reactivation on relapse after allogeneic hematopoietic cell transplantation in acute myeloid leukemia patients is influenced by conditioning regimen. Biol Blood Marrow Transplant. 2014;20:46-52.Manjappa S., Bhamidipati P.K., Stokerl-Goldstein K.E., DiPersio J.F., Uy G.L., Westervelt P. et al. Protective effect of cytomegalovirus reactivation on relapse after allogeneic hematopoietic cell transplantation in acute myeloid leukemia patients is influenced by conditioning regimen. Biol Blood Marrow Transplant. 2014;20:46-52.18. Schmidt-Hieber M., Labopin M., Beelen D., Volin L., Ehninger G., Finke J. et al. CMV serostatus still has an important prognostic impact in de novo acute leukemia patients after allogeneic stem cell transplantation: a report from the Acute Leukemia Working Party of EBMT. Blood. 2013;122:3359-3364.Schmidt-Hieber M., Labopin M., Beelen D., Volin L., Ehninger G., Finke J. et al. CMV serostatus still has an important prognostic impact in de novo acute leukemia patients after allogeneic stem cell transplantation: a report from the Acute Leukemia Working Party of EBMT. Blood. 2013;122:3359-3364.19. Teira P., Battiwalla M., Ramanathan M., Barrett A.J., Ahn K.W., Chen M. et al. Early cytomegalovirus reactivation remains associated with increased transplant-related mortality in the current era: a CIBMTR analysis. Blood. 2016;127:2427-2438.Teira P., Battiwalla M., Ramanathan M., Barrett A.J., Ahn K.W., Chen M. et al. Early cytomegalovirus reactivation remains associated with increased transplant-related mortality in the current era: a CIBMTR analysis. Blood. 2016;127:2427-2438.20. Zutter M.M., Martin P.J., Sale G.E., Shulman H.M., Fisher L., Thomas E.D. et al. Epstein-Barr virus lymphoproliferation after bone marrow transplantation. Blood. 1988;72:520-529.Zutter M.M., Martin P.J., Sale G.E., Shulman H.M., Fisher L., Thomas E.D. et al. Epstein-Barr virus lymphoproliferation after bone marrow transplantation. Blood. 1988;72:520-529.21. Lynch B.A., Vasef M.A., Comito M., Gilman A.L., Lee N., Ritchie J. et al. Effect of in vivo lymphocyte-depleting strategies on development of lymphoproliferative disorders in children post allogeneic bone marrow transplantation. Bone Marrow Transplant. 2003;32:527-533.Lynch B.A., Vasef M.A., Comito M., Gilman A.L., Lee N., Ritchie J. et al. Effect of in vivo lymphocyte-depleting strategies on development of lymphoproliferative disorders in children post allogeneic bone marrow transplantation. Bone Marrow Transplant. 2003;32:527-533.22. Micallef I.N., Chhanabhai M., Gascoyne R.D., Shepherd J.D., Fung H.C., Nantel S.H. et al. Lymphoproliferative disorders following allogeneic bone marrow transplantation: the Vancouver experience. Bone Marrow Transplant. 1998;22:981-987.Micallef I.N., Chhanabhai M., Gascoyne R.D., Shepherd J.D., Fung H.C., Nantel S.H. et al. Lymphoproliferative disorders following allogeneic bone marrow transplantation: the Vancouver experience. Bone Marrow Transplant. 1998;22:981-987.23. Handgretinger R. New approaches to graft engineering for haploidentical bone marrow transplantation. Semin Oncol. 2012;39(6):664-673.Handgretinger R. New approaches to graft engineering for haploidentical bone marrow transplantation. Semin Oncol. 2012;39(6):664-673.24. Schumm M., Lang P., Bethge W., Faul C., Feuchtinger T., Pfeiffer M. et al. Depletion of T-cell receptor alpha/beta and CD19 positive cells from apheresis products with the CliniMACS device. Cytotherapy. 2013;15(10):1253-1258.Schumm M., Lang P., Bethge W., Faul C., Feuchtinger T., Pfeiffer M. et al. Depletion of T-cell receptor alpha/beta and CD19 positive cells from apheresis products with the CliniMACS device. Cytotherapy. 2013;15(10):1253-1258.25. Muccio L., Bertaina A., Falco M., Pende D., Meazza R., Lopez-Botet M. et al. Analysis of memory-like natural killer cells in human cytomegalovirus-infected children undergoing alphabeta+T and B cell-depleted hematopoietic stem cell transplantation for hematological malignancies. Haematologica. 2016;101:371-381.Muccio L., Bertaina A., Falco M., Pende D., Meazza R., Lopez-Botet M. et al. Analysis of memory-like natural killer cells in human cytomegalovirus-infected children undergoing alphabeta+T and B cell-depleted hematopoietic stem cell transplantation for hematological malignancies. Haematologica. 2016;101:371-381.26. Knight A., Madrigal A.J., Grace S., Sivakumaran J., Kottaridis P., Mackinnon S. et al. The role of Vdelta2-negative gammadelta T cells during cytomegalovirus reactivation in recipients of allogeneic stem cell transplantation. Blood. 2010;116:2164-2172.Knight A., Madrigal A.J., Grace S., Sivakumaran J., Kottaridis P., Mackinnon S. et al. The role of Vdelta2-negative gammadelta T cells during cytomegalovirus reactivation in recipients of allogeneic stem cell transplantation. Blood. 2010;116:2164-2172.27. Farnault L., Gertner-Dardenne J., Gondois-Rey F., Michel G., Chambost H., Hirsch I. et al. Clinical evidence implicating gamma-delta T cells in EBV control following cord blood transplantation. Bone Marrow Transplant. 2013;48:1478-1479.Farnault L., Gertner-Dardenne J., Gondois-Rey F., Michel G., Chambost H., Hirsch I. et al. Clinical evidence implicating gamma-delta T cells in EBV control following cord blood transplantation. Bone Marrow Transplant. 2013;48:1478-1479.28. Scheper W., van Dorp S., Kersting S., Pietersma F., Lindemans C., Hol S. et al. gammadeltaT cells elicited by CMV reactivation after allo-SCT cross-recognize CMV and leukemia. Leukemia. 2013;27:1328-1338.Scheper W., van Dorp S., Kersting S., Pietersma F., Lindemans C., Hol S. et al. gammadeltaT cells elicited by CMV reactivation after allo-SCT cross-recognize CMV and leukemia. Leukemia. 2013;27:1328-1338.29. Airoldi I., Bertaina A., Prigione I., Zorzoli A., Pagliara D., Cocco C. et al. gd T-cell reconstitution after HLA-haploidentical hematopoietic transplantation depleted of TCR-+/CD19+ lymphocytes. Blood. 2015;125(15):2349-2358.Airoldi I., Bertaina A., Prigione I., Zorzoli A., Pagliara D., Cocco C. et al. gd T-cell reconstitution after HLA-haploidentical hematopoietic transplantation depleted of TCR-+/CD19+ lymphocytes. Blood. 2015;125(15):2349-2358.30. Przepiorka D., Weisdorf D., Martin P., Klingemann H.G., Beatty P., Hows J. et al. 1994 Consensus Conference on Acute GVHD Grading. Bone Marrow Transplant. 1995;15:825-828.Przepiorka D., Weisdorf D., Martin P., Klingemann H.G., Beatty P., Hows J. et al. 1994 Consensus Conference on Acute GVHD Grading. Bone Marrow Transplant. 1995;15:825-828.31. Goldsmith S.R., Slade M., DiPersio J.F., Westervelt P., Lawrence S.J., Uy G.L. et al. Cytomegalovirus viremia, disease, and impact on relapse in T-cell replete peripheral blood haploidentical hematopoietic cell transplantation with post-transplant cyclophosphamide. Haematologica. 2016;101(11):е465-е468.Goldsmith S.R., Slade M., DiPersio J.F., Westervelt P., Lawrence S.J., Uy G.L. et al. Cytomegalovirus viremia, disease, and impact on relapse in T-cell replete peripheral blood haploidentical hematopoietic cell transplantation with post-transplant cyclophosphamide. Haematologica. 2016;101(11):е465-е468.32. Crocchiolo R., Bramanti S., Vai A., Sarina B., Mineri R., Casari E. et al. Infections after T-replete haploidentical transplantation and high-dose cyclophosphamide as graft-versus-host disease prophylaxis. Transpl Infect Dis. 2015;17:242-249.Crocchiolo R., Bramanti S., Vai A., Sarina B., Mineri R., Casari E. et al. Infections after T-replete haploidentical transplantation and high-dose cyclophosphamide as graft-versus-host disease prophylaxis. Transpl Infect Dis. 2015;17:242-249.33. Buyck H.C., Prentice H.G., Griffiths P.D., Emery V.C. The risk of early and late CMV DNAemia associated with Campath use in stem cell transplant recipients. Bone Marrow Transplant. 2010;45:1212-1219.Buyck H.C., Prentice H.G., Griffiths P.D., Emery V.C. The risk of early and late CMV DNAemia associated with Campath use in stem cell transplant recipients. Bone Marrow Transplant. 2010;45:1212-1219.34. Huang Y.T., Neofytos D., Foldi J., Kim S.J., Maloy M., Chung D. et al. Cytomegalovirus Infection after CD34(+)-Selected Hematopoietic Cell Transplantation. Biol Blood Marrow Transplant. 2016;22:1480-1486.Huang Y.T., Neofytos D., Foldi J., Kim S.J., Maloy M., Chung D. et al. Cytomegalovirus Infection after CD34(+)-Selected Hematopoietic Cell Transplantation. Biol Blood Marrow Transplant. 2016;22:1480-1486.35. Hakki M., Riddell S.R., Storek J., Carter R.A., Stevens-Ayers T., Sudour P. et al. Immune reconstitution to cytomegalovirus after allogeneic hematopoietic stem cell transplantation: impact of host factors, drug therapy, and subclinical reactivation. Blood. 2003;102:3060-3067.Hakki M., Riddell S.R., Storek J., Carter R.A., Stevens-Ayers T., Sudour P. et al. Immune reconstitution to cytomegalovirus after allogeneic hematopoietic stem cell transplantation: impact of host factors, drug therapy, and subclinical reactivation. Blood. 2003;102:3060-3067.36. Yoon H.S., Lee J.H., Choi E.S., Seo J.J., Moon H.N., Kim M.N. et al. Cytomegalovirus infection in children who underwent hematopoietic stem cell transplantation at a single center: a retrospective study of the risk factors. Pediatr Transplant. 2009;13:898-905.Yoon H.S., Lee J.H., Choi E.S., Seo J.J., Moon H.N., Kim M.N. et al. Cytomegalovirus infection in children who underwent hematopoietic stem cell transplantation at a single center: a retrospective study of the risk factors. Pediatr Transplant. 2009;13:898-905.37. Sellar R.S., Vargas F.A., Henry J.Y., Verfuerth S., Charrot S., Beaton B. et al. CMV promotes recipient T-cell immunity following reduced-intensity T-cell-depleted HSCT, significantly modulating chimerism status. Blood. 2015;125:731-739.Sellar R.S., Vargas F.A., Henry J.Y., Verfuerth S., Charrot S., Beaton B. et al. CMV promotes recipient T-cell immunity following reduced-intensity T-cell-depleted HSCT, significantly modulating chimerism status. Blood. 2015;125:731-739.38. Moins-Teisserenc H., Busson M., Scieux C., Bajzik V., Cayuela J.M., Clave E. et al. Patterns of cytomegalovirus reactivation are associated with distinct evolutive profiles of immune reconstitution after allogeneic hematopoietic stem cell transplantation. J Infect Dis. 2008;198:818-826.Moins-Teisserenc H., Busson M., Scieux C., Bajzik V., Cayuela J.M., Clave E. et al. Patterns of cytomegalovirus reactivation are associated with distinct evolutive profiles of immune reconstitution after allogeneic hematopoietic stem cell transplantation. J Infect Dis. 2008;198:818-826.39. Lilleri D., Gerna G., Fornara C., Chiesa A., Comolli G., Zecca M. et al. Human cytomegalovirus-specific T cell reconstitution in young patients receiving T cell-depleted, allogeneic hematopoietic stem cell transplantation. J Infect Dis. 2009;199:829-836.Lilleri D., Gerna G., Fornara C., Chiesa A., Comolli G., Zecca M. et al. Human cytomegalovirus-specific T cell reconstitution in young patients receiving T cell-depleted, allogeneic hematopoietic stem cell transplantation. J Infect Dis. 2009;199:829-836.40. Lilleri D., Gerna G., Zelini P., Chiesa A., Rognoni V., Mastronuzzi A. et al. Monitoring of human cytomegalovirus and virus-specific T-cell response in young patients receiving allogeneic hematopoietic stem cell transplantation. PLoS One. 2012;7:e41648.Lilleri D., Gerna G., Zelini P., Chiesa A., Rognoni V., Mastronuzzi A. et al. Monitoring of human cytomegalovirus and virus-specific T-cell response in young patients receiving allogeneic hematopoietic stem cell transplantation. PLoS One. 2012;7:e41648.41. Link C.S., Eugster A., Heidenreich F., Rücker-Braun E., Schmiedgen M., Oelschlägel U. et al. Abundant cytomegalovirus (CMV) reactive clonotypes in the CD8(+) T cell receptor alpha repertoire following allogeneic transplantation. Clin Exp Immunol. 2016;184:389-402.Link C.S., Eugster A., Heidenreich F., Rücker-Braun E., Schmiedgen M., Oelschlägel U. et al. Abundant cytomegalovirus (CMV) reactive clonotypes in the CD8(+) T cell receptor alpha repertoire following allogeneic transplantation. Clin Exp Immunol. 2016;184:389-402.42. Hiwarkar P., Gajdosova E., Qasim W., Worth A., Breuer J., Chiesa R. et al. Frequent occurrence of cytomegalovirus retinitis during immune reconstitution warrants regular ophthalmic screening in high-risk pediatric allogeneic hematopoietic stem cell transplant recipients. Clin Infect Dis. 2014;58:1700-1706.Hiwarkar P., Gajdosova E., Qasim W., Worth A., Breuer J., Chiesa R. et al. Frequent occurrence of cytomegalovirus retinitis during immune reconstitution warrants regular ophthalmic screening in high-risk pediatric allogeneic hematopoietic stem cell transplant recipients. Clin Infect Dis. 2014;58:1700-1706.43. Barfield R.C., Otto M., Houston J., Holladay M., Geiger T., Martin J. et al. A one-step large-scale method for T- and B-cell depletion of mobilized PBSC for allogeneic transplantation. Cytotherapy. 2004;6:1-6.Barfield R.C., Otto M., Houston J., Holladay M., Geiger T., Martin J. et al. A one-step large-scale method for T- and B-cell depletion of mobilized PBSC for allogeneic transplantation. Cytotherapy. 2004;6:1-6.