B- and T-cell V(D)J-recombination excision circles as indicators of immunological reconstitution in children with acute lymphoblastic leukemia

Igor V. Obraztsov; Образцов Игорь Владимирович; Maria A. Gordukova; Гордукова Мария Александровна; Ekaterina V. Tsvetkova; Цветкова Екатерина Вадимовна; Elena V. Kononova; Кононова Елена Владиславовна; Ivan Ya. Tomilin; Томилин Иван Яковлевич; Konstantin L. Kondratchik; Кондратчик Константин Леонидович; Aleksandr F. Karelin; Карелин Александр Федорович; Andrey P. Prodeus; Продеус Андрей Петрович; Aleksandr I. Karachunskiy; Карачунский Александр Исаакович; Alexandr G. Rumyantsev; Румянцев Александр Григорьевич

doi:10.24287/1726-1708-2016-15-4-42-50

B- and T-cell V(D)J-recombination excision circles as indicators of immunological reconstitution in children with acute lymphoblastic leukemia

Authors: Obraztsov I.V.¹^,2, Gordukova M.A.³, Tsvetkova E.V.¹, Kononova E.V.⁴, Tomilin I.Y.⁵, Kondratchik K.L.⁵, Karelin A.F.¹, Prodeus A.P.³, Karachunskiy A.I.¹, Rumyantsev A.G.¹
Affiliations:
1. Dmitry Rogachev Federal Research Centre of Paediatric Haematology, Oncology and Immunology, Ministry of Health of the Russian Federation
2. A.N.Ryzhykh State Research Centre of Coloproctology, Ministry of Health of the Russian Federation
3. G.N.Speransky Children’s City Clinical Hospital No 9, Moscow Department of Health
4. Russian Cardiology Research and Production Complex, Ministry of Health of the Russian Federation
5. Morozov City Children’s Clinical Hospital, Moscow Department of Health
Issue: Vol 15, No 4 (2016)
Pages: 42-50
Section: ЛЕЙКОЗЫ
Submitted: 19.09.2018
Published: 19.12.2016
URL: https://hemoncim.com/jour/article/view/132
DOI: https://doi.org/10.24287/1726-1708-2016-15-4-42-50
ID: 132

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Abstract

Acute lymphoblastic leukemia (ALL) is the most common malignant haematological disease of childhood. ALL is associated with the development of an immune defect, promoting development of both serious infectious complications at the initial stage of therapy and long-term persistent infections in patients during remission. The work studied naîve T and B cells in 49 children in the first acute period of ALL and 55 convalescent children at different terms of remission based on detection of T-cell receptor (TRECs) and K-deleting element (KRECs) excision circles by real-time PCR. As has been shown, development of ALL itself does not lead to suppression of naîve T- and B cell maturation. Specific therapy promotes development of a deep defect of both T and B cell immunity, deficit of naîve T cells persisting for not less than three years after statement of remission, whereas maturation of В cells is restored after termination of the intensive phase of therapy. Also, the inexpediency of using high dosages of methotrexate and cranial irradiation from the viewpoint of long-term immune reconstitution has been confirmed.

Keywords

дети, острый лимфобластный лейкоз, эксцизионные кольца Т-клеточного рецептора, к-делеционный элемент, children, acute lymphoblastic leukemia, T cell receptor excision circles, k-deleting element

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B- and T-cell V(D)J-recombination excision circles as indicators of immunological reconstitution in children with acute lymphoblastic leukemia

Full Text

Abstract

Keywords

About the authors

References

Supplementary files