Primary diagnostics and assessment of minimal disseminated and minimal residual disease in Burkitt lymphoma/leukemia by detection of c-MYC-IgH rearrangements with long-distance polymerase chain reaction

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Abstract

The aim of this study was to identify с-MYC-IgH with long-distance polymerase chain reaction (LD-PCR) for Burkitt's lymphoma/ leukemia diagnosis, to compare LD-PCR with cytogenetic methods and to evaluate the possibility of LD-PCR application for minimal disseminated (MdD) and minimal residual disease (MRD) detection. с-MYC-IgH rearrangements were detected in 63,6% of the 55 patients at the age 2-23 years. In comparison to G-banding, LD-PCR was possible to perform for all patients, however LD-PCR detected с-MYC-IgH only in 71,1% of the patients with translocation t (8;14)(q24;q32). с-MYC-IgH rearrangements were found by LD-PCR in 2 of 5 (40%) of patients who were negative by FISH and G-banding. Combination of these methods allowed to reveal of c-MYC rearrangements in 94,5% of the patients. MDD was identified in 11,1% patient with stage III and in all patients with stage IV both negative and positive by standard morphological analysis. LD-PCR could be used for primary diagnosis of Burkitt's lymphoma/leukemia and MDD detection. At the same time, LD-PCR is less informative for MRD evaluation because of low sensitivity and possibility to obtain only qualitative results.

About the authors

Victoria A. Lavrinenko

Belarusian Research Center for Pediatric Oncology, Hematology and Immunology

Author for correspondence.
Email: lavrinenkovictoria@gmail.com
Russian Federation

Elena V. Volochnik

Belarusian Research Center for Pediatric Oncology, Hematology and Immunology

Email: valochnikalena@gmail.com
Russian Federation

Alina S. Fedorova

Belarusian Research Center for Pediatric Oncology, Hematology and Immunology

Email: alina_fedorova@list.ru
Russian Federation

Olga V. Aleinikova

Belarusian Research Center for Pediatric Oncology, Hematology and Immunology

Email: aleinikova2004@mail.ru
Russian Federation

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Copyright (c) 2016 Lavrinenko V.A., Volochnik E.V., Fedorova A.S., Aleinikova O.V.

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