The use of a spatial thrombin generation method for assessment of platelet procoagulant activity after platelet concentrate transfusion in children

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Abstract

Assessment of the effectiveness of platelet concentrate transfusion after hematopoietic stem cell transplantation is based on calculation of the amount of platelets and does not take into account their functional activity. The article offers an integral study of the state of hemostasis in 10 children (aged 3 to 16 years, median 11 years) before and after platelet concentrate transfusion with the use of tests for assessment of platelet functional activity and thrombin generation in space (thrombodynamics-4D). Two hours after platelet concentrate transfusion the amount of platelet increased from 8.0 ± 6.6 to 52.3 ± 25.2 X 109/L. Secretion of platelet dense granules (1.37 ± 0.48, norm 3.52 ± 1.14 units) and a-granules (5969 ± 2366, norm 11787 ± 4639 units), activated (385 ± 167, norm 1279 ± 682 units) and non-activated glycoprotein IIb/IIIa (3179 ± 1609, norm 5557 ± 2017 units) before transfusion was lower as compared with values of healthy volunteers and did not change after transfusion. A characteristic for healthy volunteers «thrombin wave» in studying thrombodynamics-4D was not formed in samples of patients both before and after transfusion. After platelet concentrate transfusion, an increase of the clot growth rate from 25 ± 8 to 32 ± 4 pm/min (p < 0.005) and thrombin reaction product from 124 ± 49 to 209 ± 38 pM*mm was observed (p < 0.001). The increased amounts of thrombin are indicative of improvement of blood coagulation after platelet concentrate transfusion, but parameters of platelet functional activity and thrombodynamics-4D do not reach normal values.

About the authors

Ekaterina M. Kol'tsova

Federal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry Rogachev

Author for correspondence.
Email: ekaterina_koltsova@bk.ru
Russian Federation

Junior Anna Balandina

Federal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry Rogachev; Centre for Theoretical Problems of Physico-Chemical Pharmacology, Russian Academy of Sciences

Email: a_balandina@inbox.ru
Russian Federation

Irina A. Demina

Federal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry Rogachev

Email: idemina@mail.ru
Russian Federation

Svetlana A. Radygina

Federal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry Rogachev

Email: svetlana.radygina@fccho-moscow.ru
Russian Federation

Fazoil I. Ataullakhanov

Federal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry Rogachev; Centre for Theoretical Problems of Physico-Chemical Pharmacology, Russian Academy of Sciences; M.V.Lomonosov Moscow State University; Moscow Institute of Physics and Technology (State University)

Email: ataullakhanov.fazly@gmail.com
Russian Federation

Dmitrii N. Balashov

Federal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry Rogachev

Email: dmitry.balashov@fccho-moscow.ru
Russian Federation

Mikhail A. Panteleev

Federal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry Rogachev; Centre for Theoretical Problems of Physico-Chemical Pharmacology, Russian Academy of Sciences; M.V.Lomonosov Moscow State University; Moscow Institute of Physics and Technology (State University)

Email: mapanteleev@yandex.ru
Russian Federation

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Copyright (c) 2016 Kol'tsova E.M., Balandina J.A., Demina I.A., Radygina S.A., Ataullakhanov F.I., Balashov D.N., Panteleev M.A.

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