Email this article A single-center experience of using immunofluorescence staining of blood smears for the diagnosis of hereditary thrombocytopathies
- Authors: Yushkova E.V.1, Podoplelova N.A.1,2, Fedorova D.V.1, Khoreva A.L.1, Shcherbina A.Y.1, Zharkov P.A.1, Panteleev M.A.1
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Affiliations:
- Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
- Center for Theoretical Problems of Physicochemical Pharmacology
- Issue: Vol 22, No 3 (2023)
- Pages: 43-47
- Section: ORIGINAL ARTICLES
- Submitted: 01.10.2023
- Accepted: 01.10.2023
- Published: 01.10.2023
- URL: https://hemoncim.com/jour/article/view/765
- DOI: https://doi.org/10.24287/1726-1708-2023-22-3-43-47
- ID: 765
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Abstract
The method of immunofluorescence staining of blood smears is a recently developed approach to the remote diagnosis of various platelet pathologies including MYH9 disorders/MYH9-related disease, biallelic Bernard–Soulier syndrome, Glanzmann thrombasthenia, gray platelet syndrome, and others. We report here the experience of introducing this method at the Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology (Moscow, Russia), which is the main national pediatric hematology hospital that provides diagnosis and therapy to children with blood disorders throughout the country. Our study aimed to transfer this relatively labor-intensive and skill-sensitive method and introduce it into routine laboratory practice, and to perform its validation. The study was approved by the Independent Ethics Committee and the Scientific Council of the Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology.
About the authors
E. V. Yushkova
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Moscow
Russian FederationN. A. Podoplelova
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation; Center for Theoretical Problems of Physicochemical Pharmacology
Author for correspondence.
ORCID iD: 0000-0001-8013-1112
Nadezhda A. Podoplelova - PhD, a leading researcher at the Laboratory of Cell Hemostasis and Thrombosis.
1 Samory Mashela St., Moscow 117997
Russian FederationD. V. Fedorova
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0003-4567-1871
Moscow
Russian FederationA. L. Khoreva
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0002-8697-4206
Moscow
Russian FederationA. Yu. Shcherbina
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0002-3113-4939
Moscow
Russian FederationP. A. Zharkov
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0003-4384-6754
Moscow
Russian FederationM. A. Panteleev
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0002-8128-7757
Moscow
Russian FederationReferences
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