Email this article Minimal residual disease monitoring in B-lineage acute lymphoblastic leukemia using flow cytometry. Guidelines of the Russian-Belarusian multicenter group for pediatric acute leukemia studies
- Authors: Popov A.M.1, Mikhailova E.V.1, Verzhbitskaya T.Y.2, Movchan L.V.3, Permikin Z.V.2, Shman T.V.3, Karachunskiy A.I.1, Novichkova G.A.1
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Affiliations:
- The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
- Regional Children's Clinical Hospital No.1
- The Belarusian Research Center for Pediatric Oncology, Hematology and Immunology
- Issue: Vol 22, No 3 (2023)
- Pages: 199-209
- Section: DIAGNOSTIC GUIDELINES
- Submitted: 03.10.2023
- Accepted: 03.10.2023
- Published: 03.10.2023
- URL: https://hemoncim.com/jour/article/view/773
- DOI: https://doi.org/10.24287/1726-1708-2023-22-3-199-209
- ID: 773
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Abstract
Multicolor flow cytometry is now routinely used in laboratory practice for minimal residual disease (MRD) monitoring in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). This article describes the methodology of MRD detection in BCP-ALL using flow cytometry as recommended by the Russian-Belarusian multicenter group for pediatric acute leukemia studies. This wellharmonized approach includes recommendations for the choice of monoclonal antibodies, sample preparation, cytometer setup, flow cytometry data analysis and interpretation as well as for reporting. These guidelines allow application of multicolor flow cytometry for MRD monitoring in BCP-ALL in children and adults both in local laboratories and in multicenter settings in prospective clinical trials.
About the authors
A. M. Popov
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Author for correspondence.
Email: uralcytometry@gmail.com
ORCID iD: 0000-0002-0889-6986
Moscow
Russian FederationE. V. Mikhailova
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0002-3450-0498
Moscow
Russian FederationT. Yu. Verzhbitskaya
Regional Children's Clinical Hospital No.1
ORCID iD: 0000-0001-9329-1828
Ekaterinburg
Russian FederationL. V. Movchan
The Belarusian Research Center for Pediatric Oncology, Hematology and Immunology
Minsk Region, Borovlyany
Russian FederationZh. V. Permikin
Regional Children's Clinical Hospital No.1
Ekaterinburg
Russian FederationT. V. Shman
The Belarusian Research Center for Pediatric Oncology, Hematology and Immunology
Minsk Region, Borovlyany
Russian FederationA. I. Karachunskiy
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Moscow
Russian FederationG. A. Novichkova
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0002-2322-5734
Moscow
Russian FederationReferences
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