Problems of flow cytometry immunophenotyping in Russia: Experience gained by the Reference Center of Moscow-Berlin Cooperative Clinical Group

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Abstract

Identification of the tumor cell immunophenotype in acute lymphoblastic leukemia (ALL) by flow cytometry is one of the priority diagnostic methods, determining the correct choice of therapy. The flow cytometry immunophenotyping standards are still not determined in Russian Federation - at the national level or at the levels of individual study groups. The groups involved in the ALL-MB-2015 study have created a reference center intended for repair of the flaws of flow cytometry immunophenotyping in the clinics involved in the study. The work of the reference center has made it possible to identify numerous problems with flow cytometer calibration, wrong choice of monoclonal antibodies and preparation of samples, incorrect analysis and interpretation of flow cytometry data. These results demonstrate very serious problems in flow cytometry immunophenotyping in Russia and complete absence of standards for this method. The basic solution for this problem is potential centralization of flow cytometry immunophenotyping at several major laboratories with experienced staff.

About the authors

A. M. Popov

Regional Pediatric Clinical Hospital No. 1; Institute of Medical Cell Technologies

Author for correspondence.
Email: uralcytometry@gmail.com
Russian Federation

S. N. Lagoiko

Federal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry Rogachev

Email: lagoiko80@mail.ru
Russian Federation

Yu. V. Rumyantseva

Federal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry Rogachev; Russian National Research Medical University named after N.I.Pirogov

Email: j.roumiantseva@mail.ru
Russian Federation

S. A. Lugovskaya

Russian Medical Academy of Postgraduate Education

Email: salugovskaya@gmail.com
Russian Federation

L. G. Fechina

Regional Pediatric Clinical Hospital No 1; Institute of Medical Cell Technologies

Email: childrens_oncology@mail.ru
Russian Federation

S. A. Rumyantsev

Federal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry Rogachev; Russian National Research Medical University named after N.I. Pirogov; Moscow Physical Technological Institute

Russian Federation

A. I. Karachunsky

Federal Research Center of Pediatric Hematology, Oncology, and Immunology named after Dmitry Rogachev; Russian National Research Medical University named after N.I.Pirogov

Email: aikarat@mail.ru
Russian Federation

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Copyright (c) 2015 Popov A.M., Lagoiko S.N., Rumyantseva Y.V., Lugovskaya S.A., Fechina L.G., Rumyantsev S.A., Karachunsky A.I.

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This work is licensed under a Creative Commons Attribution 4.0 International License.