Email this article Bone marrow cell morphology in congenital diserythropoietic anemia: selective enrichment of the studied cell population for light and electron microscopy using a microarray and centrifugation in a density gradient
- Authors: Obydennyi S.I.1, Fedyanina O.S.2, Khvastunova A.N.2, Zakirova A.O.1, Panteleev M.A.3, Kireev I.I.4, Kuznetsova S.A.2, Smetanina N.S.5
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Affiliations:
- Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology, Immunology
- Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology, Immunology Centre for Theoretical Problems of Physico-Chemical Pharmacology
- Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology, Immunology Centre for Theoretical Problems of Physico-Chemical Pharmacology M.V. Lomonosov Moscow State University
- M.V. Lomonosov Moscow State University
- Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology, Immunology Pirogov Russian National Research Medical University
- Issue: Vol 17, No 1 (2018)
- Pages: 104-107
- Section: Статьи
- Submitted: 09.08.2018
- Published: 09.02.2018
- URL: https://hemoncim.com/jour/article/view/35
- DOI: https://doi.org/10.24287/1726-1708-2018-17-1-104-107
- ID: 35
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Abstract
Congenital diserythropoietic anemias are a group of rare inherited diseases causing uneffective erythropoiesis and multiple morphological changes in erythroblasts. Their differential diagnosis involves a study of the morphology of minor bone marrow cell populations that requires a selective cell enrichment. Here we describe two methods of the population enrichment by erythroid cells (including the self-developed cell-binding microarray) for their subsequent study by methods of light and electron microscopy.
About the authors
S. I. Obydennyi
Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology, Immunology
Author for correspondence.
ORCID iD: 0000-0002-2930-8768
Russian Federation
O. S. Fedyanina
Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology, ImmunologyCentre for Theoretical Problems of Physico-Chemical Pharmacology
Russian Federation
A. N. Khvastunova
Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology, ImmunologyCentre for Theoretical Problems of Physico-Chemical Pharmacology
ORCID iD: 0000-0002-7117-0168
Russian Federation
A. O. Zakirova
Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology, ImmunologyRussian Federation
M. A. Panteleev
Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology, ImmunologyCentre for Theoretical Problems of Physico-Chemical Pharmacology
M.V. Lomonosov Moscow State University
ORCID iD: 0000-0002-8128-7757
Russian Federation
I. I. Kireev
M.V. Lomonosov Moscow State University
ORCID iD: 0000-0001-9252-6808
Russian Federation
S. A. Kuznetsova
Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology, ImmunologyCentre for Theoretical Problems of Physico-Chemical Pharmacology
Email: kuznetsova.sonya@gmail.com
ORCID iD: 0000-0001-5946-0026
Academic secretary of High school of Molecular and Experimental Medicine,
Russia 117997, Moscow, Samory Mashela st., 1
+7 (495) 287-6570
N. S. Smetanina
Dmitriy Rogachev National Medical Research Center of Pediatric Hematology, Oncology, ImmunologyPirogov Russian National Research Medical University
ORCID iD: 0000-0003-2756-7325
Russian Federation
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