Email this article The use of droplet digital polymerase chain reaction for the molecular diagnosis and monitoring of treatment response in patients with Langerhans cell histiocytosis with the BRAF V600E mutation
- Authors: Osipova D.S.1, Raykina E.V.1, Lyudovskikh E.I.1, Evseev D.A.1, Kalinina I.I.1, Baydildina D.D.1, Popov A.M.1, Semchenkova A.A.1, Burtsev E.A.1, Bronin G.O.2, Maschan A.A.1, Maschan M.A.1
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Affiliations:
- The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
- The Morozov Children's City Clinical Hospital of the Department of Health of Moscow
- Issue: Vol 22, No 1 (2023)
- Pages: 12-20
- Section: PROMISING STUDIES
- Submitted: 22.10.2022
- Accepted: 07.02.2023
- Published: 14.02.2023
- URL: https://hemoncim.com/jour/article/view/671
- DOI: https://doi.org/10.24287/1726-1708-2023-22-1-12-20
- ID: 671
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Abstract
This article discusses the potential of droplet digital polymerase chain reaction (PCR) for the diagnostic detection and monitoring of the allelic load of the BRAF V600E mutation in circulating cell-free DNA and myeloid progenitor cell population in the bone marrow of patients with Langerhans cell histiocytosis (LCH). Droplet digital PCR may serve as a useful tool for the monitoring of minimal residual disease and improve our understanding of the pathogenesis of Langerhans cells histiocytosis. The study was approved by the Independent Ethics Committee and the Scientific Council of the Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology.
About the authors
D. S. Osipova
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Author for correspondence.
Email: darya.osipova@fccho-moscow.ru
ORCID iD: 0000-0002-9968-9332
Daria S. Osipova, a research technician at the Laboratory of Molecular Biology
1 Samory Mashela St., Moscow 117997, Russia
Russian FederationE. V. Raykina
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Email: e_raikina@inbox.ru
ORCID iD: 0000-0002-7634-2053
Moscow
Russian FederationE. I. Lyudovskikh
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Email: evcola@yandex.ru
ORCID iD: 0000-0003-0717-2019
Moscow
Russian FederationD. A. Evseev
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Email: dmitryevseev1991@gmail.com
ORCID iD: 0000-0001-8610-0624
Moscow
I. I. Kalinina
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Email: burbir@mail.ru
ORCID iD: 0000-0002-0813-5626
Moscow
D. D. Baydildina
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Email: Dina.Baydildina@fccho-moscow.ru
ORCID iD: 0000-0001-7130-8596
Moscow
A. M. Popov
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Email: uralcytometry@gmail.com
ORCID iD: 0000-0002-0889-6986
Moscow
A. A. Semchenkova
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Email: sasha.semchenkova@fccho-moscow.ru
ORCID iD: 0000-0002-7082-1694
Moscow
E. A. Burtsev
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Email: burcev.evgeniy@yandex.ru
ORCID iD: 0000-0002-3623-6547
Moscow
G. O. Bronin
The Morozov Children's City Clinical Hospital of the Department of Health of Moscow
Email: gleb.bronin@ya.ru
ORCID iD: 0000-0002-0694-3996
Moscow
A. A. Maschan
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Email: Aleksey.Maschan@fccho-moscow.ru
ORCID iD: 0000-0002-0016-6698
Moscow
M. A. Maschan
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Email: mmaschan@yandex.ru
ORCID iD: 0000-0003-1735-0093
Moscow
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