Email this article Flow cytometry in acute leukemia diagnostics. Guidelines of Russian-Belarusian multicenter group for pediatric leukemia studies
- Authors: Popov A.M.1, Verzhbitskaya T.Y.2, Movchan L.V.3, Demina I.A.1, Mikhailova E.V.1, Semchenkova A.A.1, Permikin Z.V.2, Shman T.V.3, Karachunskiy A.I.1, Novichkova G.A.1
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Affiliations:
- The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
- Regional Children's Clinical Hospital No.1
- Belarusian Research Center for Pediatric Oncology, Hematology and Immunology
- Issue: Vol 22, No 1 (2023)
- Pages: 165-177
- Section: DIAGNOSTIC GUIDELINES
- Submitted: 26.01.2023
- Accepted: 01.03.2023
- Published: 14.02.2023
- URL: https://hemoncim.com/jour/article/view/704
- DOI: https://doi.org/10.24287/1726-1708-2023-22-1-165-177
- ID: 704
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Abstract
Flow cytometry is one of the key technologies for acute leukemia (AL) diagnostics. Nevertheless, lack of technological standards hampers implementation of immunophenotyping data in treatment protocols. Earlier our group published the acute lymphoblastic leukemia diagnostic standards. In this paper, we present the updated guidelines for initial immunophenotyping of ALs. This wellharmonized approach includes recommendations for monoclonal antibodies choice, sample preparation, cytometer setup, data analysis and interpretation as well as for the report writing. These guidelines allows application of diagnostic flow cytometric studies in all types of AL.
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About the authors
A. M. Popov
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Author for correspondence.
ORCID iD: 0000-0002-0889-6986
Alexander M. Popov, Cand. Med. Sci., Head of Leukemia Immunophenotyping Laboratory
1 Samory Mashela St., Moscow 117997, Russia
Russian FederationT. Yu. Verzhbitskaya
Regional Children's Clinical Hospital No.1
ORCID iD: 0000-0001-9329-1828
Ekaterinburg
Russian FederationL. V. Movchan
Belarusian Research Center for Pediatric Oncology, Hematology and Immunology
Minsk
BelarusI. A. Demina
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Moscow
Russian FederationE. V. Mikhailova
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Moscow
Russian FederationA. A. Semchenkova
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Moscow
Russian FederationZh. V. Permikin
Regional Children's Clinical Hospital No.1
Ekaterinburg
Russian FederationT. V. Shman
Belarusian Research Center for Pediatric Oncology, Hematology and Immunology
Minsk
BelarusA. I. Karachunskiy
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Moscow
Russian FederationG. A. Novichkova
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0002-2322-5734
Moscow
Russian FederationReferences
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