Email this article Guidelines for the flow cytometric minimal residual disease monitoring in B-lineage acute lymphoblastic leukemia after CD19-directed immunotherapy
- Authors: Mikhailova E.V.1, Illarionova O.I.1, Maschan M.A.1, Novichkova G.A.1, Karachunskiy A.I.1, Popov A.M.1
-
Affiliations:
- Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
- Issue: Vol 22, No 2 (2023)
- Pages: 175-184
- Section: DIAGNOSTIC GUIDELINES
- Submitted: 06.04.2023
- Accepted: 25.05.2023
- Published: 08.07.2025
- URL: https://hemoncim.com/jour/article/view/734
- DOI: https://doi.org/10.24287/1726-1708-2023-22-2-175-184
- ID: 734
Cite item
Full Text
Abstract
Multicolor flow cytometry is now routinely used in laboratory practice for the minimal residual disease (MRD) monitoring in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Wide application of CD19-directed immunotherapy leads to frequent loss of CD19 expression, that hampers significantly the flow cytometric MRD detection methodology. We developed an antibody panel and data analysis algorithm for multicolor flow cytometry, which is a reliable method for MRD detection in patients with BCP-ALL treated with CD19-directed therapy. We recommend a single-tube 11-color panel for MRD detection, which is adapted for the case of possible CD19 loss. Based on patterns of antigen expression changes and the relative expansion of normal CD19-negative BCPs, guidelines for multicolored flow cytometry data analysis and interpretation are established. The recommended approach is reliable tool for therapy response monitoring displaying the same effectiveness with the more laborious and costly molecular techniques.
About the authors
E. V. Mikhailova
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Author for correspondence.
Email: katmikhailova1805@gmail.com
ORCID iD: 0000-0002-3450-0498
Ekaterina V. Mikhailova, MD
Leukemia Immunophenotyping Laboratory
117997
1 Samory Mashela St.
Moscow
Russian FederationO. I. Illarionova
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0003-2685-674X
Moscow
Russian FederationM. A. Maschan
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0003-1735-0093
Moscow
Russian FederationG. A. Novichkova
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0002-2322-5734
Moscow
Russian FederationA. I. Karachunskiy
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
Moscow
Russian FederationA. M. Popov
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation
ORCID iD: 0000-0002-0889-6986
Moscow
Russian FederationReferences
Supplementary files
